000103621 001__ 103621 000103621 005__ 20230824003322.0 000103621 02470 $$2DOI 000103621 0247_ $$2doi$$a10.7936/6rxs-103621 000103621 037__ $$aRDM 000103621 041__ $$aeng 000103621 245__ $$aRobust acoustic trapping and perturbation of single-cell microswimmers illuminate three-dimensional swimming and ciliary coordination [dataset] 000103621 260__ $$bWashington University in St. Louis 000103621 269__ $$a2023-06-05 000103621 270__ $$mmeachamjm@wustl.edu$$pJ. Mark Meacham 000103621 336__ $$aCollection 000103621 518__ $$d2019-01-01/2022-12-31$$oCollected 000103621 520__ $$aThis collection comprises raw data and media files supporting the PNAS manuscript, "Robust acoustic trapping and perturbation of single-cell microswimmers illuminate three-dimensional swimming and ciliary coordination." The unicellular alga Chlamydomonas reinhardtii is widely used to investigate the mechanisms underlying ciliary beating and coordination. Freely swimming cells are difficult to image with sufficient resolution to capture cilia motion, necessitating that the cell body be held during experiments. Acoustic confinement enables investigation of cell and cilia motion under conditions that approximate free swimming. The video files in this repository entry are used to measure cell and cilia behavior under acoustically trapped and free swimming conditions. 000103621 536__ $$oNational Science Foundation$$qhttps://ror.org/021nxhr62$$rROR$$cCBET-1944063$$aCAREER: Dynamic characterization of acoustofluidic devices using living probes$$1https://www.nsf.gov/awardsearch/showAward?AWD_ID=1944063 000103621 536__ $$oNational Science Foundation$$qhttps://ror.org/021nxhr62$$rROR$$cCMMI-1633971$$aCharacterizing dynamic transitions and bifurcations to understand how flagella beat$$1https://www.nsf.gov/awardsearch/showAward?AWD_ID=1633971 000103621 540__ $$aCreative Commons Zero (CC0) 1.0 Universal$$uhttps://creativecommons.org/publicdomain/zero/1.0/ 000103621 650__ $$aPhysical sciences 000103621 650__ $$aBiological sciences 000103621 650__ $$aMechanical engineering 000103621 6531_ $$amicroswimmers 000103621 6531_ $$acilia 000103621 6531_ $$aflagella 000103621 6531_ $$aacoustofluidics 000103621 6531_ $$aacoustic tweezers 000103621 655__ $$aVideo 000103621 655__ $$aTabular 000103621 655__ $$aText 000103621 7001_ $$aMeacham, J. Mark$$1https://orcid.org/0000-0002-3124-0707$$uWashington University in St. Louis$$4https://ror.org/01yc7t268$$5ROR 000103621 7001_ $$aCui, Mingyang$$1https://orcid.org/0000-0002-9610-5725$$uMassachusetts Institute of Technology$$4https://ror.org/042nb2s44$$5ROR 000103621 7001_ $$aDutcher, Susan$$1https://orcid.org/0000-0001-5689-5753$$uWashington University in St. Louis$$4https://ror.org/01yc7t268$$5ROR 000103621 7001_ $$aBayly, Philip$$1https://orcid.org/0000-0003-4303-0704$$uWashington University in St. Louis$$4https://ror.org/01yc7t268$$5ROR 000103621 720__ $$aMeacham, J. Mark$$uWashington University in St. Louis$$1https://orcid.org/0000-0002-3124-0707$$4https://ror.org/01yc7t268$$5ROR$$eProjectLeader$$7Personal 000103621 720__ $$aCui, Mingyang$$uMassachusetts Institute of Technology$$1https://orcid.org/0000-0002-9610-5725$$4https://ror.org/042nb2s44$$5ROR$$eProjectMember$$7Personal 000103621 791__ $$tCui M, Dutcher SK, Bayly PV, Meacham JM. "Robust acoustic trapping and perturbation of single-cell microswimmers illuminate three-dimensional swimming and ciliary coordination." Proceedings of the National Academy of Sciences (PNAS) June 16, 2023. 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