TY  - GEN
AB  - Diabetes cell replacement therapy has the potential to be transformed by human pluripotent stem cell-derived β cells (SC-β cells). However, the precise identity of SC-β cells in relationship to primary fetal and adult β-cells remains unclear. Here, we used single-cell sequencing datasets to characterize the transcriptional identity of islets from in vitro differentiation, fetal islets, and adult islets. Our analysis revealed that SC-β cells share a core β-cell transcriptional identity with human adult and fetal β-cells, however SC-β cells possess a unique transcriptional profile characterized by the persistent expression and activation of progenitor and neural-biased gene networks. These networks are present in SC-β cells, irrespective of the derivation protocol used. Notably, fetal β-cells also exhibit this neural signature at the transcriptional level. Our findings offer insights into the transcriptional identity of SC-β cells and underscore the need for further investigation of the role of neural transcriptional networks in their development.
AD  - Washington University in St. Louis
AD  - Washington University in St. Louis
AD  - Washington University in St. Louis
AD  - Washington University in St. Louis
AU  - Schmidt, Mason
AU  - Ishahak, Matthew
AU  - Augsornworawat, Punn
AU  - Millman, Jeffrey
DA  - 2024-02-23
DO  - 10.7936/6rxs-103656
DO  - doi
ID  - 103656
KW  - Biological sciences
KW  - Basic medicine
KW  - Health sciences
KW  - Health biotechnology
KW  - SC-beta
KW  -  scRNA-seq 
KW  - Transcriptome 
KW  - Gene networks
KW  - Diabetes
KW  - Immaturity
L1  - https://data.library.wustl.edu/record/103656/files/all_endocrine.rds
L1  - https://data.library.wustl.edu/record/103656/files/all_beta.rds
L1  - https://data.library.wustl.edu/record/103656/files/readme_doi1079366rxs103656_ishahak.txt
L2  - https://data.library.wustl.edu/record/103656/files/all_endocrine.rds
L2  - https://data.library.wustl.edu/record/103656/files/all_beta.rds
L2  - https://data.library.wustl.edu/record/103656/files/readme_doi1079366rxs103656_ishahak.txt
L4  - https://data.library.wustl.edu/record/103656/files/all_endocrine.rds
L4  - https://data.library.wustl.edu/record/103656/files/all_beta.rds
L4  - https://data.library.wustl.edu/record/103656/files/readme_doi1079366rxs103656_ishahak.txt
LA  - eng
LK  - https://data.library.wustl.edu/record/103656/files/all_endocrine.rds
LK  - https://data.library.wustl.edu/record/103656/files/all_beta.rds
LK  - https://data.library.wustl.edu/record/103656/files/readme_doi1079366rxs103656_ishahak.txt
N2  - Diabetes cell replacement therapy has the potential to be transformed by human pluripotent stem cell-derived β cells (SC-β cells). However, the precise identity of SC-β cells in relationship to primary fetal and adult β-cells remains unclear. Here, we used single-cell sequencing datasets to characterize the transcriptional identity of islets from in vitro differentiation, fetal islets, and adult islets. Our analysis revealed that SC-β cells share a core β-cell transcriptional identity with human adult and fetal β-cells, however SC-β cells possess a unique transcriptional profile characterized by the persistent expression and activation of progenitor and neural-biased gene networks. These networks are present in SC-β cells, irrespective of the derivation protocol used. Notably, fetal β-cells also exhibit this neural signature at the transcriptional level. Our findings offer insights into the transcriptional identity of SC-β cells and underscore the need for further investigation of the role of neural transcriptional networks in their development.
PB  - Washington University in St. Louis
PY  - 2024-02-23
T1  - Single-cell RNA Sequencing Analysis of Stem Cell-derived Beta Cells
TI  - Single-cell RNA Sequencing Analysis of Stem Cell-derived Beta Cells
UR  - https://data.library.wustl.edu/record/103656/files/all_endocrine.rds
UR  - https://data.library.wustl.edu/record/103656/files/all_beta.rds
UR  - https://data.library.wustl.edu/record/103656/files/readme_doi1079366rxs103656_ishahak.txt
Y1  - 2024-02-23
ER  -